Despite significant advances in the field applications of reserve electrodialysis (RED) to produce salinity gradient power, net energy production remains an issue owing to limitations such as high energy requirement for high flow rates of feed solutions, and severe fouling and pressure build up when thin spacers are used. Therefore, to maximize the performance and efficiency of energy harvesting in the RED, a cascaded RED stack, with multiple stages between the anode and cathode electrodes, was investigated. In cascaded stacks, 100-cell paired stacks were divided into several stages, so the feed water flowed into the first stage, and the effluent from the first stage was then reused in the next stages. This cascaded stack could overcome the typical drawbacks of RED (large amount of feed water required, intensive pumping energy, and low net energy production). Although 25% of the feed water volume was used in the 4-stage cascaded stack (100-cell-pairs) compared to the conventional stack (100-cell-pairs with a parallel flow operation), much more energy was produced with the 4-stage cascaded stack. The net power density and net specific energy with the 4-stage cascaded stack were the highest at 0.5 cm/s (0.48 W/m2) and 0.25 cm/s (0.06 kWh/m3), respectively. This is very promising for the practical application of RED since feed water volumes can be greatly reduced, which could reduce the burden on the feed water pretreatment step. Consequently, we can build a compact RED plant with smaller pretreatment processes and fewer RED unit stacks.