Velocity profiles in tidal channels cause cyclic oscillations in hydrodynamic loads due to the dependence of relative velocity on angular position, which can lead to fatigue damage. Therefore, the effect of velocity profile on the load variation and fatigue life of large-scale tidal turbines is quantified here. This is accomplished using Fluid Structure Interaction (FSI) simulations created using the ANSYS Workbench software, which couples the fluid solver ANSYS CFX to the structural solver ANSYS transient structural. While these load oscillations only minimally impact power and thrust fluctuation for rotors, they can significantly impact the load variations on individual rotor blades. To evaluate these loadings, a tidal turbine within a channel with a representative flow that follows a 1/7th power velocity profile and an onset turbulence intensity of 5% is simulated. This velocity profile increases the thrust coefficient variation from mean cycle value of an individual blade from 2.8% to 9% and the variation in flap wise bending moment coefficient is increased from 4.9% to 19%. Similarly, the variation from the mean cycle value for blade deformation and stress of 2.5% and 2.8% increased to 9.8% and 10.3%, respectively. Due to the effect of velocity profile, the mean stress is decreased, whereas, the range and variation of stress are considerably increased.