Innovation in materials and test protocols, as well as physical and numerical investigations, is required to address the technical challenges arising due to the novel application of components from conventional industries to the marine renewable energy (MRE) industry. Synthetic fibre ropes, widely used for offshore station-keeping, have potential application in the MRE industry to reduce peak mooring line loads. This paper presents the results of a physical characterisation study of a novel hybrid polyester-polyolefin rope for MRE mooring applications through a round robin testing (RRT) approach at two test facilities. The RRT was performed using standard guidelines for offshore mooring lines and the results are verified through the numerical modelling of the rope tensile behaviour. The physical testing provides quantifiable margins for the strength and stiffness properties of the hybrid rope, increases confidence in the test protocols and assesses facility-specific influences on test outcomes. The results indicate that the adopted guidance is suitable for rope testing in mooring applications and there is good agreement between stiffness characterisation at both facilities. Additionally, the numerical model provides a satisfactory prediction of the rope tensile behaviour and it can be used for further parametric studies.